Heterochromatic Repeat Clustering Imposes a Physical Barrier on Homologous Recombination to Prevent Chromosomal Translocations
نویسندگان
چکیده
Mouse pericentromeric DNA is composed of tandem major satellite repeats, which are heterochromatinized and cluster together to form chromocenters containing homologous sequences from different chromosomes. These clusters protected damage refractory repair through recombination (HR). Moreover, double stranded breaks (DSBs) occurring within relocate away the core domain be repaired. The mechanisms by pericentric heterochromatin imposes a barrier on HR implications repeat clustering unknown. Here, we compare spatial recruitment factors upon DSBs induced specifically in human mouse heterochromatin, differ their capacity clusters. We show that while increase accessibility disrupting HP1 α dimerization, physical requires many layers decompaction accessed. Our results consistent with model 3D organization heterochromatic regions dictates activation pathways key prevent clustered repeats onset chromosomal translocations.
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ژورنال
عنوان ژورنال: Social Science Research Network
سال: 2021
ISSN: ['1556-5068']
DOI: https://doi.org/10.2139/ssrn.3796494